Project A11 - Dieter Bruns

Complexin and synaptotagmin in Ca2+-triggered exocytosis

The project investigates the action of CpxII and its interplay with the major Ca2+ sensors Synaptotagmin I and VII in Ca2+-triggered exocytosis of chromaffin cells. Starting from the corresponding null mutants (single and double null mutants) and in the framework of detailed structure-function analysis, we aim to achieve this goal by using a combination of electrophysiological, biochemical, and fluorescence microscopic techniques (confocal/STED).

Furthermore, we will study CpxII’s role in gliotransmission from astrocytes and will investigate its impact on integrative functions of hippocampal signaling.