Ca2+ signals:
Molecular Mechanisms and Integrative Functions Collaborative Research Center 894

Project A9 - Ute Becherer

Docking and priming of large dense-core vesicles in neurons and neuroendocrine cells 

Ca2+-dependent secretion of large dense core vesicles (LDCVs) appears to follow different rules in chromaffin cells and in dorsal root ganglion (DRG) neurons. We will focus our investigation on the molecular mechanism of dead-end docking in chromaffin cells. In DRG neurons, we will examine the role of priming factors and we will be especially interested in secretion at growth cones. We will assess the role of these factors in chromaffin cells and in DRG neurons with over-expression, deletion and rescue experiments using various methods such as TIRFM, FRET and BiFC, but also live confocal fluorescence microscopy.