Ca2+ signals:
Molecular Mechanisms and Integrative Functions Collaborative Research Center 894

Project A3 - Veit Flockerzi & Stephan Philipp

CaVβ2- and CaVβ3-specific signal transduction

The CaVβ-subunits are associated with the ion-conducting α1 pore of voltage-gated CaV channels and are indispensable for α1-targeting to the plasma membrane and modulation of CaV channel properties. Furthermore, they are also associated with non-CaV channel proteins serving functions not directly related to CaV channel activity. We plan to identify novel proteins interacting with the CaVβ2 and CaVβ3 and characterize the function of these protein complexes. The CaVβ-subunits are encoded by four genes and splicing of these genes generates numerous splice variants, especially for CaVβ2. The precise function of the single splice variants is not known and by generating mice expressing the two predominant β2 splice variants, β2-N1 or β2-N4, as transgenes in CaVβ2-/- mice we intend to characterize splice variant-specific CaVβ2-functions in vivo.